Expression of a cytokinin synthesis gene from Agrobacterium tumefaciens T-DNA in basket willow (Salix viminalis)

Willow cells transformed with an ipt gene from Agrobacterium tumefaciens grow in tissue culture as undifferentiated callus without shoot induction. We show that the transformed calluses contained high levels of the cytokinins 9-β- d -ribofuranosyl zeatin and its monophosphate, demonstrating the presence of a functional isopentenyl transferase enzyme. The ipt gene was transcribed at different levels in different transformed callus lines. The absence of shoot differntiation is apparently not due to a lack of zeatin-type cytokinins in the transformed callus.     

Sex and season-related differences of basic haematological values in the mountain reedbuck (Reduncula fulvorufula).

1. Basic haematological values in the peripheral blood of four male and 13 female mountain reedbucks (Reduncula fulvorufula) have been repeatedly estimated. 2. Comparing these results for both sexes, we noted pronouncedly lower haematocrit values and haemoglobin contents in females (statistical significance less than or greater than 1%). In the white blood cells only an increased proportion of neutrophils in females was apparent. 3. We have been unable to find seasonal related differences in the red and white blood overall picture of the cells during the summer and winter seasons with the exception of raised red cell counts and increased proportion of monocytes in the winter. The presented findings are compared and discussed with those abstracted from the literature.